DNA TECHNOLOGİES
DNA technology has revolutionized modern science. Deoxyribonucleic acid (DNA), or an organism's genetic material—inherited from one generation to the next—holds many clues that have unlocked some of the mysteries behind human behavior, disease, evolution, and aging. As technological advances lead to a better understanding of DNA, new DNA-based technologies will emerge. Recent advances in DNA technology including cloning, PCR, recombinant DNA technology, DNA fingerprinting, gene therapy, DNA microarray technology, and DNA profiling have already begun to shape medicine, forensic sciences, environmental sciences, and national security.
In 1956, the structure and composition of DNA was elucidated and confirmed previous studies more than a decade earlier demonstrating DNA is the genetic material that is passed down from one generation to the next. A novel tool called PCR (polymerase chain reaction) was developed not long after DNA was descovered. PCR represents one of the most significant discoveries or inventions in DNA technology and it lead to a 1993 Nobel Prize award for American born Kary Mullis (1949–).
PCR is the amplification of a specific sequence of DNA so that it can be analyzed by scientists. Amplification is important, particularly when it is necessary to analyze a small sequence of DNA in quantities that are large enough to perform other molecular analyses such as DNA sequencing. Not long after PCR technology was developed, genetic engineering of DNA through recombinant DNA technology quickly became possible. Recombinant DNA is DNA that has been altered using bacterial derived enzymes called restriction endonucleases that act like scissors to cut DNA. The pattern that is cut can be matched to a pattern cut by the same enzymes from a different DNA sequence. The sticky ends that are created bind to each other and a DNA sequence can therefore be inserted into another DNA sequence.
Restriction endonucleases are also important in genetic fingerprinting. In this case, enzymes that recognize specific DNA sequences can produce fragments of DNA by cutting different parts of a long strand of DNA. If there are differences in the sequence due to inherited variation—meaning that there are extra DNA or specific sequences altered such that the restriction enzymes no longer recognize the site, variable patterns can be produced. If these patterns are used to compare two different people, they will have a different fragment pattern or fingerprint. Genetic fingerprinting can be used to test for paternity. In forensics, genetic fingerprinting can be used to identify a criminal based on whether their unique DNA sequence matches to DNA extracted from a crime scene. This technology can also allow researchers to produce genetic maps of chromosomes based on these restriction enzyme fingerprints. Becasue there are many different enzymes, many different fingerprints can be ascertained.

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